Protocols
This page contains our the finalized versions of our field & lab protocols for this project. Our intention is to release each of our protocols in both document & video format. If you have a question about how we do things, or a suggestion or comment on our methods, please reach us at our contact page.
Sampling Protocols
Sampling Kit Prep and Water Sample Collection Protocol
This protocol outlines our methods for preparing for, and collecting eDNA water samples in the field : eDNA Water Sampling Protocol. The videos below demonstrate Sampling Kit Prep and Sample Collection as described in the sampling protocol.
Water sample Filtering lab Protocol
This covers our protocol for filtering water samples in preparation for extraction. See our tutorial video at right, or download the protocol below.
At some sites it may be preferable to filter while in the field. We have a brief recommended protocol here:
Lab Protocols
DNA Extraction with Kingfisher
Qubit (DNA Concentration) measurement
12S PCR for Fish Species (MiFish) optimized for turbid systems
